Purification of mycobacterial deoxyribonucleic acid.

Gene Expression Systems and Recombinant Protein Purification

There is no substance as important as deoxyribonucleic acid as it carries within its own structure the hereditary in formations that determines the structure of proteins, the important molecule of life. Deoxyribonucleic acid transcribe messenger ribonucleic acid which finally translates protein the essential macromolecules. Through gene expression the information contained in gene can be utiliz...

Dark repair of ultraviolet-irradiated deoxyribonucleic acid by bacteriophage T4: purification and characterization of a dimer-specific phage-induced endonuclease.

The purification and properties of an ultraviolet (UV) repair endonuclease are described. The enzyme is induced by infection of cells of Escherichia coli with phage T4 and is missing from extracts of cells infected with the UV-sensitive and excision-defective mutant T4V(1). The enzyme attacks UV-irradiated deoxyribonucleic acid (DNA) containing either hydroxymethylcytosine or cytosine, but does...

Enzymatic Synthesis of Deoxyribonucleic Acid. Xv. Purification and Properties of a Polymerase from Bacillus Subtilis.

Characterization of the deoxyribonucleic acid of various strains of halophilic bacteria.

Bacteria classified as extreme halophiles, in the genera Halobacterium and Halococcus, contain deoxyribonucleic acid (DNA) which displays two components in a CsCl equilibrium density gradient. The base composition of the major DNA component ranges from 66 to 68% guanine plus cytosine (GC), whereas that of the satellite DNA comprising some 11 to 36% of the total, is between 57 and 60% GC. Purifi...

The Enzymatic Methylation of Ribonucleic Acid and Deoxyribonucleic Acid V. PURIFICATION AND PROPERTIES OF THE DEOXYRIBONUCLEIC ACID-METHYLATING ACTIVITY OF ESCHERICHIA COLP

The classical investigations of Wyatt (2) and Dunn and Smith (3) established the existence of 5-methylcytosine and 6-methylaminopurine as constituents of the deoxyribonucleic acid of higher plants and animals, and of bacteria and bacterial viruses, respectively. Until recently, the biochemical pathways for the incorporation of these “trace bases” into deoxyribonucleic acid was unknown although ...